貨號(hào) PH0612

名稱 Tris-乙酸電泳緩沖液（50×TAE）

規(guī)格 500mL

存儲(chǔ) 室溫儲(chǔ)存，有效期至少12 個(gè)月。

產(chǎn)品簡(jiǎn)介 

TAE 是生物學(xué)中使用最廣泛的核酸電泳緩沖液，主要用于 DNA 的瓊脂糖凝膠電泳。TAE 的主要成分是 Tris-乙酸鹽與 EDTA，電泳時(shí)雙鏈線狀 DNA 的遷移率較快，電泳大于 13kb 的片段時(shí)分離效果好，此外，回收DNA 片段時(shí)也宜用 TAE 緩沖系統(tǒng)進(jìn)行電泳。

TAE是使用最廣泛的緩沖系統(tǒng)。其特點(diǎn)是超螺旋在其中電泳時(shí)更符合實(shí)際相對(duì)分子質(zhì)量（TBE中電泳時(shí)測(cè)出的相對(duì)分子質(zhì)量會(huì)大于實(shí)際分子質(zhì)量），且雙鏈線狀DNA在其中的遷移率較其他兩種緩沖液快約10%，電泳大于13kb的片段時(shí)用TAE緩沖液將取得更好的分離效果，此外，回收DNA片段時(shí)也易用TAE緩沖系統(tǒng)進(jìn)行電泳。TAE的缺點(diǎn)是緩沖容量小，長時(shí)間電泳（如過夜）不可選用，除非有循環(huán)裝置使兩極的緩沖液得到交換。TAE是使用最廣泛的緩沖系統(tǒng)。其特點(diǎn)是超螺旋在其中電泳時(shí)更符合實(shí)際相對(duì)分子質(zhì)量（TBE中電泳時(shí)測(cè)出的相對(duì)分子質(zhì)量會(huì)大于實(shí)際分子質(zhì)量），且雙鏈線狀DNA在其中的遷移率較其他兩種緩沖液快約10%，電泳大于13kb的片段時(shí)用TAE緩沖液將取得更好的分離效果。TAE的缺點(diǎn)是緩沖容量小，長時(shí)間電泳（如過夜）不可選用，除非有循環(huán)裝置使兩極的緩沖液得到交換。

TBE的特點(diǎn)是緩沖能力強(qiáng)，長時(shí)間電泳時(shí)可選用TBE，并且當(dāng)用于電泳小于1kb的片段時(shí)分離效果更好。TBE用于瓊脂糖凝膠時(shí)易造成高電滲作用，并且因與瓊脂糖相互作用生成非共價(jià)結(jié)合的四羥基硼酸鹽復(fù)合物而使DNA片段的回收率降低，所以不宜在回收電泳中使用。

本產(chǎn)品為 50×濃縮液，工作濃度為 1×，可直接用蒸餾水稀釋 50 倍后使用。若產(chǎn)品出現(xiàn)沉淀析出，請(qǐng)置于37℃水浴使其溶解，不影響使用。

注意事項(xiàng) 

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TAE buffer is a buffer solution containing a mixture of Tris base, acetic acid and EDTA.

In molecular biology it is used in agarose electrophoresis typically for the separation of nucleic acids such as DNA and RNA. It is made up of Tris-acetate buffer, usually at pH 8.3, and EDTA, which sequesters divalent cations. TAE has a lower buffer capacity than TBE and can easily become exhausted, but linear, double stranded DNA runs faster in TAE.TAE (Tris-acetate-EDTA) buffer is used as both a running buffer and in agarose gel. Its use in denaturing gradient gel electrophoresis methods for broad-range mutation analysis has also been described. TAE has been used at various concentrations to study the mobility of DNA in solution with and without sodium chloride. However, high concentrations of sodium chloride (and many other salts) in a DNA sample retard its mobility. This may lead to incorrect interpretations of the resulting DNA banding pattern.